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Isolation of Quorum Sensing Inhibitory compounds from cyanobacteria
MACHO, Markéta
2020 - English
Overuse of antibiotics has led to the development of resistance in many medically relevant human pathogens, posing a threat to human health. The recent decline in the discovery of novel antibiotics together with development of multi-antibiotic resistant strains demands a search of an alternative approach. Anti-virulence therapy poses significantly lower pressure on developing resistance given its focus on disarming the pathogen rather than eradicating it. Advancement in the field of bacterial cell-to-cell communication (Quorum Sensing, QS) and its molecular mechanism regulating the production of virulence factors in many clinically relevant human pathogens led to the discovery of Quorum Sensing inhibitory (QSI) molecules. Cyanobacteria are recognized as a prolific source of natural bioactive compounds with great pharmacological potential. The aim of this study was to screen cyanobacterial extracts for QSI activity in search of potential anti-virulence drugs. 45 cyanobacterial strains were randomly selected from the in-house culture collection of Centre Algatech, extracted, and fractionated to generate 1575 fractions for QSI evaluation. Strains 3, 16, and 113 exhibited the highest inhibitory potential against both used biosensors, E. coli pSB401 and E. coli pSB1075. The obtained results open a future prospect to isolate and elucidate the lead molecule responsible for QSI activity.
Keywords:
Cyanobacteria; Quorum sensing inhibition; Antimicrobial resistance; Anti-virulence therapy; Anti-microbial activity; Hemolytic activity
Available in the Digital Repository of University of South Bohemia.
Isolation of Quorum Sensing Inhibitory compounds from cyanobacteria
Overuse of antibiotics has led to the development of resistance in many medically relevant human pathogens, posing a threat to human health. The recent decline in the discovery of novel antibiotics ...
Francie jako obchodní partner Visegrádské skupiny
BATSUURI, Nomin
2021 - English
The topic of this bachelor's thesis is France as a business partner of the Visegrád group. The aim of the thesis is to assess the foreign trade between France and the Visegrád group and to define the main development trends. The theoretical part of the thesis deals with the common sense of foreign trade, determination of certain fundamental conceptions concerning foreign trade and the description of chosen theories based on literature research. In the practical part, it attends to particular facts in the field of foreign trade of the countries of the Visegrád group and France and multilateral foreign trade between these countries. In the conclusion of the thesis, the observations about the development of foreign trade between France and the Visegrád group are summarized, as well as the factors affecting mutual trade. Tématem bakalářské práce je Francie jako obchodní partner Visegrádské skupiny. Cílem práce je posouzení vzájemného obchodního partnerství zemí Visegrádské skupiny s Francií. V teoretické části se práce zabývá obecným pojetím zahraničního obchodu, vymezením některých zásadních pojmů týkajících se zahraničního obchodu a popisem vybraných teorií na základě literární rešerše. V praktické části se práce věnuje konkrétním faktům z oblasti zahraničního obchodu zemí Visegrádské skupiny a Francie a vzájemného zahraničního obchodu mezi těmito zeměmi. V závěru jsou shrnuty poznatky o vývoji zahraničního obchodu mezi Francií a Visegrádskou skupinou, také faktory ovlivňující vzájemnou obchodní výměnu.
Keywords:
zahraniční obchod; export; import; globalizace; obchodní bariéry; obchodní balance
Available in the Digital Repository of University of South Bohemia.
Francie jako obchodní partner Visegrádské skupiny
The topic of this bachelor's thesis is France as a business partner of the Visegrád group. The aim of the thesis is to assess the foreign trade between France and the Visegrád group and to define the ...
Characterisation of new diplonemid species via electron microscopy, starvation experiments and immunofluorescence studies
CIMRHANZLOVÁ, Kristýna
2020 - English
The large abundance and diversity of diplonemids in oceans has been overlooked until recently. Diplonemids are now starting to be recognised as an abundant and diverse group of the planktonic community. It is in our best interest to study these fascinating microorganisms to learn more about how they impact the ecosystems of the world's oceans. This thesis is focused primarily on morphological descriptions and determination of the life stages of four newly discovered species. The large abundance and diversity of diplonemids in oceans has been overlooked until recently. Diplonemids are now starting to be recognised as an abundant and diverse group of the planktonic community. It is in our best interest to study these fascinating microorganisms to learn more about how they impact the ecosystems of the world's oceans. This thesis is focused primarily on morphological descriptions and determination of the life stages of four newly discovered species.
Keywords:
diplonemids; morphology; endosymbionts; taxonomy; microscopy; immunofluorescence; species; growth curves; mitochondria; starvation experiments
Available in the Digital Repository of University of South Bohemia.
Characterisation of new diplonemid species via electron microscopy, starvation experiments and immunofluorescence studies
The large abundance and diversity of diplonemids in oceans has been overlooked until recently. Diplonemids are now starting to be recognised as an abundant and diverse group of the planktonic ...
Characterization of Trypanosoma brucei MICOS subunit Mic20.
DEISENHAMMER, Sebastian
2021 - English
This study focuses on the trypanosome MICOS complex related membrane protein Tb927.10.11900 (TbMic20) in Trypanosoma brucei. This research aimed to express TbMic20 in E. coli cells under soluble conditions and further determine its standard redox potential. This study focuses on the trypanosome MICOS complex related membrane protein Tb927.10.11900 (TbMic20) in Trypanosoma brucei. This research aimed to express TbMic20 in E. coli cells under soluble conditions and further determine its standard redox potential.
Keywords:
trypanosoma brucei; recombinant protein; mitochondria; redox potential
Available in the Digital Repository of University of South Bohemia.
Characterization of Trypanosoma brucei MICOS subunit Mic20.
This study focuses on the trypanosome MICOS complex related membrane protein Tb927.10.11900 (TbMic20) in Trypanosoma brucei. This research aimed to express TbMic20 in E. coli cells under soluble ...
Phycobilin pigments as building blocks of artificial light-harvesting structures
MALINOVÁ, Kateřina
2020 - English
Phycobilin mixture was extracted from Thermosynechococcus elongatus cyanobacteria and purified with TLC method. Pure phycocyanobilin was esterified by Fischer esterification by several alcohol types. The results were analysed by TLC and HPLC methods. Phycobilin mixture was extracted from Thermosynechococcus elongatus cyanobacteria and purified with TLC method. Pure phycocyanobilin was esterified by Fischer esterification by several alcohol types. The results were analysed by TLC and HPLC methods.
Keywords:
phycobilins; aggregates; HPLC; TLC; esterification; aggregates
Available in the Digital Repository of University of South Bohemia.
Phycobilin pigments as building blocks of artificial light-harvesting structures
Phycobilin mixture was extracted from Thermosynechococcus elongatus cyanobacteria and purified with TLC method. Pure phycocyanobilin was esterified by Fischer esterification by several alcohol types. ...
Investigation of stability and dynamics of photosynthetic pigment - protein complexes using optical spectroscopy
ODERMATT, Mirjam
2020 - English
Isolated violaxanthin-chlorophyll a binding protein of Nannochloropsis oceanica was studied by optical spectroscopy, putting a specific focus on time-resolved fluorescence spectroscopy. Thereby, dynamic and kinetic information about the pigment-protein interactions with respect to non-photochemical quenching was collected utilizing pigment and intrinsic tryptophan fluorescence. Using this method, the investigation of the molecular environment of the pigment-protein complex during the process of qE was possible. Isolated violaxanthin-chlorophyll a binding protein of Nannochloropsis oceanica was studied by optical spectroscopy, putting a specific focus on time-resolved fluorescence spectroscopy. Thereby, dynamic and kinetic information about the pigment-protein interactions with respect to non-photochemical quenching was collected utilizing pigment and intrinsic tryptophan fluorescence. Using this method, the investigation of the molecular environment of the pigment-protein complex during the process of qE was possible.
Keywords:
violaxanthin-chlorophyll a protein; VCP; non-photochemical quenching; NPQ; aggregation of LHC; light-harvesting complexes; intrinsic tryptophan fluorescence; time-resolved fluorescence; optical spectroscopy; pigment-protein complexes
Available in the Digital Repository of University of South Bohemia.
Investigation of stability and dynamics of photosynthetic pigment - protein complexes using optical spectroscopy
Isolated violaxanthin-chlorophyll a binding protein of Nannochloropsis oceanica was studied by optical spectroscopy, putting a specific focus on time-resolved fluorescence spectroscopy. Thereby, ...
Expressing Colour in Language
MARKYTÁNOVÁ, Tereza
2021 - English
This thesis aims to examine the relation between language and colour. Namely, it focuses on expressing the perception of colour as a salient feature in the process of naming mushrooms. The work is based on onomasiological approach that proceeds in its examination of the naming process from the concept itself. The colour referring terms were sorted into concrete categories of type of designation based on the way they express colour (e.g. by directly using colour terms, metaphorical expressions). The names of mushrooms were analysed and contrasted in English and Czech language. Tato práce si klade za cíl prozkoumat vztah jazyka a barvy. Konkrétně se soustředí na jazykové vyjadřování vjemu barvy jako salientního rysu při pojmenovávání hub. Práce využívá onomaziologického přístupu, který ve svém zkoumání vychází přímo od pojmenovávaného konceptu. K barvě odkazující termíny byly třízeny do konkrétních skupin typů pojmenování dle způsobu, jakým byla barva vyjádřena (např. přímé použití termínu pro barvu, metaforické pojmenování). Názvy hub byly analyzovány a kontrastovány v anglickém a českém jazyce.
Keywords:
vyjádření barvy; barevné termíny; onomasiologie; jména hub; proces pojmenovávání; metafora
Available in the Digital Repository of University of South Bohemia.
Expressing Colour in Language
This thesis aims to examine the relation between language and colour. Namely, it focuses on expressing the perception of colour as a salient feature in the process of naming mushrooms. The work is ...
Understanding protein function prediction using deep learning
SHALABY, Fathy
2021 - English
Exploring the effect of auxiliary tasks regarding the performance of a model regarding predicting the main class in the space of protein function prediction by gene ontology ids.
Keywords:
Deep Learning; Auxillary Task; Gene Ontology
Available in the Digital Repository of University of South Bohemia.
Understanding protein function prediction using deep learning
Exploring the effect of auxiliary tasks regarding the performance of a model regarding predicting the main class in the space of protein function prediction by gene ontology ids.
COMMUNICATION OF CHILDREN AT PLAY: ANALYSIS OF AUTHENTIC DATA RECORDED IN THREE IRISH FAMILIES
BOLINOVÁ, Tereza
2016 - English
The focus of the thesis is communication of three five-year-old children at play. The data were gathered at three related families, then transcribed and analysed. The first part of the work introduces the topic and the speakers. It is followed by methodology, which explains the application of Conversation analysis on the data. The third part of the thesis contains the analysis of talk and intereaction of the three children. The results of observations and a comparison of the strategies in talk are summarised and discussed in the conclusion. The aim of this work is to observe and describe frequent occurrances in the talk of children and tho give an insight into their way of thinking. This outcome could be useful when communicating with a child and possibly help with choosing the right strategy in interaction with them. Předmětem bakalářské práce je komunikace tří pětiletých dětí při hře. Zvuková data pro analýzu byla pořízena u tří příbuzných rodin, poté byla přepsána do písemné podoby a analyzována. První část práce představuje téma a mluvčí, následuje přiblížení postupu práce s daty. Pro výzkum jsou využity postupy konverzační analýzy, díky níž lze do hloubky zkoumat řeč a komunikaci. Třetí část obsahuje samotnou analýzu řeči a interakce dětí. V závěru jsou shrnuty výsledky pozorování, a srovnání postupů jejich komunikace. Cílem práce je zmapovat časté jevy v komunikaci dětí, a nastínit způsob jejich myšlení. Tyto výsledky by mohly napomoci při jednání s dětmi a podpořit vzájemné pochopení a zaujetí vhodných postupů v komunikaci.
Keywords:
komunikace; děti; konverzační analýza; interakce
Available in the Digital Repository of University of South Bohemia.
COMMUNICATION OF CHILDREN AT PLAY: ANALYSIS OF AUTHENTIC DATA RECORDED IN THREE IRISH FAMILIES
The focus of the thesis is communication of three five-year-old children at play. The data were gathered at three related families, then transcribed and analysed. The first part of the work introduces ...
Analýza finanční pomoci Afghánistánu
SIDDIQUI, Ajmal
2014 - English
This thesis is focused on foreign aid and its effect on the life of the public from 2002 until 2012 in Afghanistan. The aims of this thesis were to show the relationships among variables and the dependence on Foreign Aid at the National level and Provincial level, and, also, to show whether or not foreign aid has helped the public to increase their general living standards. The thesis looked at the ten top provinces that received the highest amount of aid and analysis was made to show the relationships between Foreign Aid verses the Number of Doctors in public Hospitals, the Amount of Transportation Money and the Number of Public and Private universities in the following provinces: Balkh, Ghazni, Heart, Helmand, Kabul, Kandahar, Kunar, Nangarhar, Paktika and Paktya. This bachelor thesis was divided into separated parts. The first part describes the theoretical background and way of gaining necessary information and also explains the most important words, which are important for understanding the whole concept of the thesis. It includes historical information about foreign aid, political information and economical information about the country. The second part defines the methodology of this thesis, which is the quantitative research method and explains the methods which were used in the practical part. The last part of this thesis describes the practical part,, which is the analysis of simple linear regression and normality test done in the STATSITCA software by using the data provided and the models were interpreted to give the final result of the thesis. It can be concluded, after analysis and interpretation of the models, that three of the models have sense and the fourth one doesn't have sense. The three models with sense show that the Number of Doctors in Public Hospitals and the Number of Public and Private universities have a strong relationship with Foreign Aid. This means that, from 2002 until 2012, foreign aid helped and improved the life of the public on a national level and a provincial level and increased their general living standards. The fourth model is the Amount of Transportation Money and it can be seen quite clearly that it has a weak relationship with Foreign aid. This means that, from 2002 until 2012, foreign aid was not so effective in this sector due to the identified reasons of: security challenges, corruption in the government offices and sub-contracting the projects. Overall, it can be said that foreign aid was clearly a major driving force in the improvement of the lives of the general public in Afghanistan. Tato práce se zaměřuje na finanční pomoc ze zahraničí a její dopady na veřejný život v Afghánistánu v letech 2002 až 2012. Cílem této práce bylo provedení analýzy této pomoci a určení odlišností a vzájemných vztahů jak na úrovni národní, tak i na úrovní jednotlivých provincií. Práce měla ukázat, na základě vybraných parametrů, do jaké míry zahraniční pomoc pomohla veřejnosti zlepšit své základní životní podmínky. Práce se zabývá těmi provinciemi, které obdržely největší částku z dané finanční pomoci, a analýza měla ukázat souvislosti zahraniční pomoci vzhledem k počtu doktorů ve veřejných nemocnicích, celkovou výši peněz investovaných do dopravní infrastruktury a počet veřejných i soukromých univerzit v následujících provinciích: Balkh, Ghazni, Heart, Helmand, Kabul, Kandahar, Kunar, Nangarhar, Paktika a Paktya. Tato bakalářská práce byla rozdělena do několika částí. První část popisuje teoretické pozadí i zdroje nezbytných informací pro provedení analýzy, a stejně tak objasňuje nejdůležitější výrazy, které jsou důležité pro pochopení celého konceptu této práce. Také obsahuje historické informace o zahraniční pomoci a politické a ekonomické informace o Afganistánu. Druhá část definuje metodologii této práce, popisuje použité kvantitativní výzkumné metoda, a vysvětluje jejich konkrétní použití v části praktické. Poslední částí práce část praktická, ve které je pro analýzu dat použita jednoduchá lineární regrese a test normality provedený v softwaru STATISTICA. Pomocí těchto metod a vytvořených modelů byla získaná data interpretována a byly formulovány závěry práce. Po analýze a interpretaci se dá usoudit, že tři modely dávaly smysl a čtvrtý nikoli. Ty tři smysluplné modely ukázaly, že počet doktorů ve veřejných nemocnicích a počet veřejných i soukromých univerzit mají silnou souvislost se zahraniční pomocí. Z toho vyplývá, že mezi lety 2002 až 2012 zahraniční pomoc pomohla a zdokonalila veřejný život na národní i provinční úrovni a rovněž zlepšila základní životní podmínky. Čtvrtý model je obnos převedených peněz, a je poměrně zřetelné, že to má slabou souvislost se zahraniční pomocí. To znamená, že v letech 2002 až 2012 nebyla zahraniční pomoc v tomto odvětví až tak efektivní, a díky poznaným důvodům to je kvůli korupci na vládní úrovni, bezpečnostním výzvám a přehodnocování projektů. Tak jako tak se dá říci, že zahraniční pomoc byla jasně hlavní tažnou silou zdokonalování veřejného života v Afghánistánu.
Keywords:
jednoduchá lineární regrese; počet doktorů ve veřejných nemocnicích; celková výše peněz investovaných do dopravní infrastruktury; počet veřejných a soukromých univerzit
Available in the Digital Repository of University of South Bohemia.
Analýza finanční pomoci Afghánistánu
pomoci, a analýza měla ukázat souvislosti zahraniční pomoci vzhledem k počtu doktorů ve veřejných nemocnicích, celkovou výši peněz investovaných do dopravní infrastruktury a počet veřejných i ...
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