Dynamics of digestive proteolytic system during blood feeding of the hard tick Ixodes ricinus
FRANTOVÁ, Helena
2011 - anglický Dynamics of the main digestive peptidases involved in digestion of host blood in the hard tick Ixodes ricinus were studied. Gene expression profiles of the particular enzymes were determined by qRT-PCR. Enzyme activities were determined using specific fluorescent probes. Midgut morphology during blood feeding was observed and immunolocalization of the main abundant peptidase - Cathepsin B - was performed. Klíčová slova: Ixodes ricinus; digestion; peptidases; immunolocalization. Plné texty jsou dostupné v digitálním repozitáři JČU. Dynamics of digestive proteolytic system during blood feeding of the hard tick Ixodes ricinus

Dynamics of the main digestive peptidases involved in digestion of host blood in the hard tick Ixodes ricinus were studied. Gene expression profiles of the particular enzymes were determined by ...

FRANTOVÁ, Helena
Jihočeská univerzita v Českých Budějovicích, 2011

Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization
ŠTĚRBOVÁ, Jarmila
2011 - anglický The main aim of this study is the biochemical characterization of a carrier protein from the ornate sheep tick Dermacentor marginatus, hemelipoglycoprotein, with emphasis on its size in native conditions, its glycosylation and identification of its modifying glycans, and examining its carbohydrate-binding specificity. The main aim of this study is the biochemical characterization of a carrier protein from the ornate sheep tick Dermacentor marginatus, hemelipoglycoprotein, with emphasis on its size in native conditions, its glycosylation and identification of its modifying glycans, and examining its carbohydrate-binding specificity. Klíčová slova: hemelipoclycoprotein; tick; Dermacentor marginatus; sugar-binding protein Plné texty jsou dostupné v digitálním repozitáři JČU. Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization

The main aim of this study is the biochemical characterization of a carrier protein from the ornate sheep tick Dermacentor marginatus, hemelipoglycoprotein, with emphasis on its size in native ...

ŠTĚRBOVÁ, Jarmila
Jihočeská univerzita v Českých Budějovicích, 2011

Contributions to the ecology of hollow dependent saproxylic beetles
ŠEBEK, Pavel
2015 - anglický The thesis consists of two studies contributing to knowledge about the ecology of two model saproxylic species dependent on tree hollows. It includes, firstly, an analysis of the habitat requirements of the violet click beetle, Limoniscus violaceus, and secondly, an analysis of the association of several saproxylic beetles with the presence of the hermit beetle, Osmoderma barnabita, in hollows. The thesis consists of two studies contributing to knowledge about the ecology of two model saproxylic species dependent on tree hollows. It includes, firstly, an analysis of the habitat requirements of the violet click beetle, Limoniscus violaceus, and secondly, an analysis of the association of several saproxylic beetles with the presence of the hermit beetle, Osmoderma barnabita, in hollows. Klíčová slova: tree cavity; endangered species; Natura 2000; insect ecology Plné texty jsou dostupné v digitálním repozitáři JČU. Contributions to the ecology of hollow dependent saproxylic beetles

The thesis consists of two studies contributing to knowledge about the ecology of two model saproxylic species dependent on tree hollows. It includes, firstly, an analysis of the habitat requirements ...

ŠEBEK, Pavel
Jihočeská univerzita v Českých Budějovicích, 2015

Fragment analysis represents a suitable approach for the detection of hotspot c.7541_7542delCT NOTCH1 mutation in chronic lymphocytic leukemia
VONKOVÁ, Barbara
2018 - anglický Chronic lymphocytic leukemia is the most common type of leukemia in adults in western countries. Despite the continual improvement of diagnostic methods and treatment, it still remains incurable. One of the main characteristic features of CLL is its clinical and biological variability. In present, the analysis of prognostic genetic markers is the most useful tool for understanding biology and clinical course of the disease and consequently the individual type of treatment. The presence of a NOTCH1 mutation is connected with shortened survival and resistance to conventional chemo-immunotherapy. In this thesis, we examined CLL patients with c.7541_7542delCT NOTCH1 mutation and the most sensitive and specific method was identified. We compared nowadays most commonly used methods: allele-specific PCR, fragment analysis and Sanger sequencing. Chronic lymphocytic leukemia is the most common type of leukemia in adults in western countries. Despite the continual improvement of diagnostic methods and treatment, it still remains incurable. One of the main characteristic features of CLL is its clinical and biological variability. In present, the analysis of prognostic genetic markers is the most useful tool for understanding biology and clinical course of the disease and consequently the individual type of treatment. The presence of a NOTCH1 mutation is connected with shortened survival and resistance to conventional chemo-immunotherapy. In this thesis, we examined CLL patients with c.7541_7542delCT NOTCH1 mutation and the most sensitive and specific method was identified. We compared nowadays most commonly used methods: allele-specific PCR, fragment analysis and Sanger sequencing. Klíčová slova: CLL; NOTCH1 mutation; fragment analysis; sequencing; allele-specific PCR Plné texty jsou dostupné v digitálním repozitáři JČU. Fragment analysis represents a suitable approach for the detection of hotspot c.7541_7542delCT NOTCH1 mutation in chronic lymphocytic leukemia

Chronic lymphocytic leukemia is the most common type of leukemia in adults in western countries. Despite the continual improvement of diagnostic methods and treatment, it still remains incurable. One ...

VONKOVÁ, Barbara
Jihočeská univerzita v Českých Budějovicích, 2018

Victims of agricultural intensification: Mowing date affects Rhinanthus spp. regeneration and fruit ripening.
BLAŽEK, Petr
2018 - anglický The recent population declines of annual hemiparasitic Rhinanthus species may be a result of changes in mowing dates associated with the intensification of grassland management, but the causal mechanisms are not well understood. This thesis describes the dynamics of Rhinanthus spp. regeneration after cutting and of fruit ripening under silage or hay making, and suggest recommendations for conservation of the target species. The recent population declines of annual hemiparasitic Rhinanthus species may be a result of changes in mowing dates associated with the intensification of grassland management, but the causal mechanisms are not well understood. This thesis describes the dynamics of Rhinanthus spp. regeneration after cutting and of fruit ripening under silage or hay making, and suggest recommendations for conservation of the target species. Klíčová slova: Yellow rattle; Hemiparasite; Agri-environmental schemes; Agricultural intensification; Delayed mowing. Plné texty jsou dostupné v digitálním repozitáři JČU. Victims of agricultural intensification: Mowing date affects Rhinanthus spp. regeneration and fruit ripening.

The recent population declines of annual hemiparasitic Rhinanthus species may be a result of changes in mowing dates associated with the intensification of grassland management, but the causal ...

BLAŽEK, Petr
Jihočeská univerzita v Českých Budějovicích, 2018

Adipokinetic hormone activities in insect body infected by entomopathogenic nematode
IBRAHIM, Emad Ahmed Sayed
2018 - anglický The role of adipokinetic hormone (AKH) in the firebug Pyrrhocoris apterus adults infected by the entomopathogenic nematode (EPN) Steinernema carpocapsae was examined in this study. It was found that co-application of EPN and AKH enhanced firebug mortality about 2.5 times within 24 h (from 20 to 51% in EPN vs. EPN + AKH treatments), and resulted in metabolism intensification, as carbon dioxide production in firebugs increased about 2.1 and 1.6 times compared to control- and EPN-treated insects, respectively. Accordingly, firebugs with reduced expression of AKH receptors showed a significantly lower mortality (by 1.6 to 2.9-folds), and lower general metabolism after EPN + AKH treatments. In addition, EPN application increased Akh gene expression in the corpora cardiaca (1.6 times), AKH level in the corpora cardiaca (1.3 times) and haemolymph (1.7 times), and lipid and carbohydrate amounts in the haemolymph. Thus, the outcomes of the present study demonstrate involvement of AKH into the antistress reaction elicited by the nematobacterial infection. The exact mechanism by which AKH acts is unknown, but results suggested that the increase of metabolism and nutrient amounts in haemolymph might play a role. The role of adipokinetic hormone (AKH) in the firebug Pyrrhocoris apterus adults infected by the entomopathogenic nematode (EPN) Steinernema carpocapsae was examined in this study. It was found that co-application of EPN and AKH enhanced firebug mortality about 2.5 times within 24 h (from 20 to 51% in EPN vs. EPN + AKH treatments), and resulted in metabolism intensification, as carbon dioxide production in firebugs increased about 2.1 and 1.6 times compared to control- and EPN-treated insects, respectively. Accordingly, firebugs with reduced expression of AKH receptors showed a significantly lower mortality (by 1.6 to 2.9-folds), and lower general metabolism after EPN + AKH treatments. In addition, EPN application increased Akh gene expression in the corpora cardiaca (1.6 times), AKH level in the corpora cardiaca (1.3 times) and haemolymph (1.7 times), and lipid and carbohydrate amounts in the haemolymph. Thus, the outcomes of the present study demonstrate involvement of AKH into the antistress reaction elicited by the nematobacterial infection. The exact mechanism by which AKH acts is unknown, but results suggested that the increase of metabolism and nutrient amounts in haemolymph might play a role. Klíčová slova: Mortality; Akh gene expression; AKH receptor; Metabolism; RNAi; Pyrrhocoris apterus Plné texty jsou dostupné v digitálním repozitáři JČU. Adipokinetic hormone activities in insect body infected by entomopathogenic nematode

The role of adipokinetic hormone (AKH) in the firebug Pyrrhocoris apterus adults infected by the entomopathogenic nematode (EPN) Steinernema carpocapsae was examined in this study. It was found that ...

IBRAHIM, Emad Ahmed Sayed
Jihočeská univerzita v Českých Budějovicích, 2018

Origin of Spergularia ×kurkae, a hybrid between the rare endemic S. echinosperma and its widespread congener S. rubra
KÚR, Pavel
2017 - anglický S využitím molekulárních metod byl zkoumán původ druhu Spergularia ×kurkae, domnělého tetraploidního křížence mezi diploidním středoevropským endemitem S. echinosperma a běžným tetraploidním druhem S. rubra. Sekvence regionu ITS (internal transcribed spacer) potvrdily hybridní původ S. ×kurkae jakožto křížence mezi S. echinosperma a S. rubra. Jelikož u téměř všech jedinců S. ×kurkae byla pozorována neúplná homogenizace rodičovských homeologů (incomplete concerted evolution), lze předpokládat, že S. ×kurkae je mladým taxonem. Je pravděpodobné, že tento taxon vznikl lidským přičiněním jakožto následek introdukce druhu S. rubra na obnažená rybniční dna. The origin of Spergularia ×kurkae, a presumed tetraploid hybrid between diploid central-European endemic S. echinosperma and its widespread tetraploid congener S. rubra, was investigated using molecular tools. Sequencing of the internal transcribed spacer (ITS) confirmed the hybrid origin of S. ×kurkae and the parentage. As incomplete concerted evolution of the ITS region was observed in nearly all individuals of S. ×kurkae, it is probable that S. ×kurkae is a young taxon. We speculate that it might have evolved as a result of human-mediated introduction of S. rubra into fishponds. Klíčová slova: endemismus; hybridizace; genová introgrese; Spergularia Plné texty jsou dostupné v digitálním repozitáři JČU. Origin of Spergularia ×kurkae, a hybrid between the rare endemic S. echinosperma and its widespread congener S. rubra

S využitím molekulárních metod byl zkoumán původ druhu Spergularia ×kurkae, domnělého tetraploidního křížence mezi diploidním středoevropským endemitem S. echinosperma a běžným tetraploidním druhem S. ...

KÚR, Pavel
Jihočeská univerzita v Českých Budějovicích, 2017

Cryptosporidium testudinis sp. n., Cryptosporidium ducismarci Traversa, 2010 and Cryptosporidium tortoise genotype III (Apicomplexa: Cryptosporidiidae) in tortoises
JEŽKOVÁ, Jana
2017 - anglický Plné texty jsou dostupné v digitálním repozitáři JČU. Cryptosporidium testudinis sp. n., Cryptosporidium ducismarci Traversa, 2010 and Cryptosporidium tortoise genotype III (Apicomplexa: Cryptosporidiidae) in tortoises

JEŽKOVÁ, Jana
Jihočeská univerzita v Českých Budějovicích, 2017

Assessment of phosphorus associated with Fe and Al (hydr)oxides in sediments and soils
JAN, Jiří
2016 - anglický The established fractionation scheme of Psenner and Pucsko with consecutive BD and NaOH steps was shown to be ineffective in distinguishing between the stable crystalline (hydr)oxides and their more active amorphous and poorly crystalline forms. These forms highly vary in their ability to sorb P and thus in their ecological significance. Dissolution kinetics of the Fe and Al minerals were performed using both the BD and NaOH extraction solutions. The results were applied to modify the original fractionation scheme for better quantification of P associated with active Al and Fe (hydr)oxides, and better estimation of the amounts of these Fe and Al forms in soils and sediments. The established fractionation scheme of Psenner and Pucsko with consecutive BD and NaOH steps was shown to be ineffective in distinguishing between the stable crystalline (hydr)oxides and their more active amorphous and poorly crystalline forms. These forms highly vary in their ability to sorb P and thus in their ecological significance. Dissolution kinetics of the Fe and Al minerals were performed using both the BD and NaOH extraction solutions. The results were applied to modify the original fractionation scheme for better quantification of P associated with active Al and Fe (hydr)oxides, and better estimation of the amounts of these Fe and Al forms in soils and sediments. Klíčová slova: phosphorus; iron; aluminum; fractionation; sediment; soil Plné texty jsou dostupné v digitálním repozitáři JČU. Assessment of phosphorus associated with Fe and Al (hydr)oxides in sediments and soils

The established fractionation scheme of Psenner and Pucsko with consecutive BD and NaOH steps was shown to be ineffective in distinguishing between the stable crystalline (hydr)oxides and their more ...

JAN, Jiří
Jihočeská univerzita v Českých Budějovicích, 2016

Tree-rings mirror management legacy: dramatic response of standard oaks to past coppicing in Central Europe?
ALTMAN, Jan
2016 - anglický Plné texty jsou dostupné v digitálním repozitáři JČU. Tree-rings mirror management legacy: dramatic response of standard oaks to past coppicing in Central Europe?

ALTMAN, Jan
Jihočeská univerzita v Českých Budějovicích, 2016