Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody
Řezáčová, Pavlína; Lescar, J.; Brynda, Jiří; Fábry, Milan; Bentley, G. A.; Sedláček, Juraj
2003 - anglický The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in the low nanomolar range. This antibody cross-reacts with peptides that include theN-terminus of the enzyme (residues 1-7), a region which is highly conserved in sequence amongdifferent viral strains and which, furthermore, is crucial for homodimerization to the activeenzymatic form.We report here two crystal structures of a recombinant single-chain Fv fragment of mAb 1696,expressed in E. coli, as a complex with a cross-reactive peptides from the HIV-1 PR and theHIV-2 PR at 2.7 ? resolution and 1.9 ? resolution respectively. On the basis of the interactionsseen in the complex three-dimensional structures, the cross-reactivity between mAb 1696 withthe HIV-1 and HIV-2 protease and their N-terminal peptides can be explained. In addition, acandidate mechanism of HIV PR inhibition by mAb 1696 is proposed which may help thedesign of alternative HIV protease inhibitors, aimed at dissociating the homodimeric viral enzyme. Klíčová slova: antibody fragment; crystal structure; HIV protease inhibition Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody

The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in ...

Řezáčová, Pavlína; Lescar, J.; Brynda, Jiří; Fábry, Milan; Bentley, G. A.; Sedláček, Juraj
Ústav molekulární genetiky, 2003

ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA
Jelínková, Petra; Liberda, J.; Ryšlavá, H.; Tichá, M.; Jonáková, Věra
2003 - anglický The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of D-mannose-containing saccharide structures and heparin may also play an important role in the sperm release from the oviductal reservoir and in the capacitation process. Klíčová slova: mannan-binding proteins; oviductal reservoir; boar seminal plasma Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA

The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of ...

Jelínková, Petra; Liberda, J.; Ryšlavá, H.; Tichá, M.; Jonáková, Věra
Ústav molekulární genetiky, 2003

Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE
Pavlásek, J.; Pěknicová, Jana; Ulčová-Galová, Z.; Nováková, P.; Mičanová, Z.; Rokyta, Z.
2003 - anglický We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples. Klíčová slova: acrosome reaction; monoclonal antibody Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Significance of the acrosome reaction and sperm agglutinating antibodies in human semen. acrosome RE

We commpared results of the acrosome reaction and sperm agglutinating antibodies in human semen samples.

Pavlásek, J.; Pěknicová, Jana; Ulčová-Galová, Z.; Nováková, P.; Mičanová, Z.; Rokyta, Z.
Ústav molekulární genetiky, 2003

Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra; Tichá, M.; Jonáková, Věra
2003 - anglický Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present communication we have shown that serine proteinase inhibitors participate (under physiological conditions) in the formation of associated complexes with other proteins from boar seminal plasma. Klíčová slova: boar seminal plasma proteins; proteinase inhibitors Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Characterization of peptide proteinase inhibitors isolated from boar seminal plasma

Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present ...

Jelínková, Petra; Tichá, M.; Jonáková, Věra
Ústav molekulární genetiky, 2003

Protein Characterization of Boar Epididymal Fluid
Maňásková, Pavla; Tichá, M.; Jonáková, Věra
2003 - anglický Klíčová slova: epididymal maturation, proteins, fertilization Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Protein Characterization of Boar Epididymal Fluid

Maňásková, Pavla; Tichá, M.; Jonáková, Věra
Ústav molekulární genetiky, 2003

Role of sperm surface proteins in gamete binding
Jonáková, Věra; Tichá, M.
2003 - anglický Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the fertilization process. Klíčová slova: boar seminal plasma proteins, gamete binding, fertilization Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Role of sperm surface proteins in gamete binding

Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the ...

Jonáková, Věra; Tichá, M.
Ústav molekulární genetiky, 2003

Study of sperm proteins in modelspecies of chondrostean and teleostean fish
Koubek, Pavel; Elzeinová, Fatima; Linhart, O.; Pěknicová, Jana
2003 - anglický Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different encironment and their protection against the outer environment is much weaker than that of oocytes. The way of fertilization also differs from mammalian fertilization. Klíčová slova: cyprinus; carpio; acipenser Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Study of sperm proteins in modelspecies of chondrostean and teleostean fish

Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different ...

Koubek, Pavel; Elzeinová, Fatima; Linhart, O.; Pěknicová, Jana
Ústav molekulární genetiky, 2003

Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Moore, H. D. M.
2003 - anglický The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes. Klíčová slova: cytoskeleton; spermatozoa Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Dynamic and role of cytosekeletal proteins in mammalian sperm cells

The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor ...

Paleček, J.; Dvořáková, K.; Pěknicová, Jana; Kubátová, Alena; Moore, H. D. M.
Ústav molekulární genetiky, 2003

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Teplá, O.; Pěknicová, Jana; Mrázek, M.; Kočí, K.; Chládek, David; Míka, M.; Badalová, J.; Pecka, T.; Fulka, F.
2003 - anglický Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted laboratory techniques supported good results from last year. The medical problem is solved during the other field of application-histlogical findings and immunofluorescent methods. Klíčová slova: MESA; azoospermia; TESE Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. noTitle

Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted ...

Teplá, O.; Pěknicová, Jana; Mrázek, M.; Kočí, K.; Chládek, David; Míka, M.; Badalová, J.; Pecka, T.; Fulka, F.
Ústav molekulární genetiky, 2003

Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte
Kyselová, Vendula; Pěknicová, Jana
2003 - anglický Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now represent a functionally related family of regulatory proteins containing a conserved PKA binding domain and a unique targeting sequence that direct the PKA-AKAP complex to subcellular structures. Klíčová slova: akap; acrosome Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR. Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte

Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now ...

Kyselová, Vendula; Pěknicová, Jana
Ústav molekulární genetiky, 2003