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Polycyklické aromatické uhlovodíky v PM1 aerosolu na 6 lokalitách v České republice
Křůmal, Kamil; Mikuška, Pavel
2019 - český
PAHs byly měřeny v zimní sezóně ve městech Ostrava-Radvanice (průmyslová lokalita), Brno, Mladá Boleslav, Kladno-Švermov, Čelákovice, Košetice (pozaďová lokalita). Z koncentrací PAHs v PM1 aerosolu byly počítány rizika vzniku rakoviny plic. PAHs were measured in winter seasons in cities/towns Ostrava-Radvanice (industrial city), Brno, Mladá Boleslav, Kladno-Švermov, Čelákovice, Košetice (background town). Lifetime lung cancer risks of PAHs were calculated from concentrations of PAHs in PM1 aerosol.
Klíčová slova:
PAHs; PM1; lung cancer risk
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Polycyklické aromatické uhlovodíky v PM1 aerosolu na 6 lokalitách v České republice
PAHs byly měřeny v zimní sezóně ve městech Ostrava-Radvanice (průmyslová lokalita), Brno, Mladá Boleslav, Kladno-Švermov, Čelákovice, Košetice (pozaďová lokalita). Z koncentrací PAHs v PM1 aerosolu ...
Microfluidic device for cell counting and electrical lysis
Václavek, Tomáš; Křenková, Jana; Foret, František
2018 - anglický
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical platforms based on visual control over the sample input, secondly a unit for electrical lysis of cells using direct current voltage pulses to lyse cells one by one with a possibility to analyze the distinct lysate zones by electrospray ionization mass spectrometry.
Klíčová slova:
microfluidic devices; microextraction of basic drugs; single cell proteomic analysis; electrospray ionization mass spectrometry; cell lysis
Dokument je dostupný na externích webových stránkách.
Microfluidic device for cell counting and electrical lysis
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical ...
Impact of post-harvest processing, geographical locality and production year on quality of selected medicinal plants
Burdějová, Lenka; Polovka, M.; Pořízka, J.
2018 - anglický
This study deals with the assessment of the impact of post-harvest treatment, geographical location and production years on 32 selected characteristics of medicinal plants grown in the Czech Republic. Dimethyl sulfoxide herbal extracts characteristics were evaluated by spectroscopic and chromatographic techniques. The results were processed by analysis of variance and multivariate statistical tools. Obtained results clearly proved the successful differentiation of herbal samples by means of canonical discrimination analysis according to post-harvest treatment (100%), geographical origin (82.9%) and production year (96.1%) confirming that studied factors may have significant effect on quality of medicinal plants.
Klíčová slova:
HPLC-DAD; ICP-OES; plants extracts; herbal extracts
Dokument je dostupný na externích webových stránkách.
Impact of post-harvest processing, geographical locality and production year on quality of selected medicinal plants
This study deals with the assessment of the impact of post-harvest treatment, geographical location and production years on 32 selected characteristics of medicinal plants grown in the Czech Republic. ...
A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
Ďurč, P.; Lačná, J.; Dosedělová, V.; Foret, František; Homoľa, L.; Pokojová, E.; Malá, M.; Skřičková, J.; Dastych, M.; Vinohradská, H.; Dřevínek, P.; Skalická, V.; Kubáň, Petr
2018 - anglický
A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. The collected sample is analyzed by capillary electrophoresis with contactless conductometric detection (CE-C4D). Ion ratios are determined as ratios of peak area of chloride anion and peak areas of selected cations. It is possible to distinguish between cystic fibrosis patients and healthy individuals when ion ratio is applied as diagnostic factor. In here, we present analytical parameters of developed method (limits of detection, limits of quantification, repeatability of migration times, repeatability of peak areas) and ROC curve, which reveals high sensitivity and specificity of the developed method.
Klíčová slova:
method for diagnostic of cystic fibrosis; cystic fibrosis; capillary electrophoresis with contactless conductometric detection
Dokument je dostupný na externích webových stránkách.
A Validatation of non-invasive method for diagnostic of cystic fibrosis based on ion ratios determined by CE-C4D
A validation of novel approach for diagnosis of cystic fibrosis (CF) is presented. The method is very simple and fast, since for collection of samples, only wiping of the skin of forearm is needed. ...
Acetonitrile-assisted enzymatic digestion used for analysis of proteins of cancer origin
Laštovičková, Markéta; Bobál, P.; Strouhalová, Dana; Bobálová, Janette
2018 - anglický
In this study, in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system was developed to facilitate bottom-up proteomic identification of proteins isolated from human breast cancer cells.
Klíčová slova:
breast cancer cells; enzymatic digestion; MS analysis
Dokument je dostupný na externích webových stránkách.
Acetonitrile-assisted enzymatic digestion used for analysis of proteins of cancer origin
In this study, in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system was developed to facilitate bottom-up proteomic identification of proteins isolated from human breast cancer ...
Optimization of photochemical vapor generation of molybdenum as a sample introduction for ICP-MS
Šoukal, Jakub; Musil, Stanislav
2018 - anglický
Conditions of photochemical vapor generation of molybdenum with ICP-MS detection have been in detail investigated.
Klíčová slova:
ICP-MS; molybdenum; photochemical vapor generation
Plné texty jsou dostupné na jednotlivých ústavech Akademie věd ČR.
Optimization of photochemical vapor generation of molybdenum as a sample introduction for ICP-MS
Conditions of photochemical vapor generation of molybdenum with ICP-MS detection have been in detail investigated.
Droplet-based microfluidic chip with passive mixer for analysis with photon-upconversion nanoparticles
Křivánková, Jana; Přikryl, Jan; Hlaváček, Antonín
2018 - anglický
Mixing of the fluids in microfluidic chips is important especially in applications, where droplet contents need to be rapidly homogenized. Here, a passive method for mixing in polydimethylsiloxane (PDMS) microchannels is presented. Droplet homogenization was firstly tested with organic stains and subsequently applied for mixing water dispersions of photon-upconversion nanoparticles (UCNPs).\n
Klíčová slova:
microfluidic chips; microfluidic systems; photon-upconversion nanoparticles
Dokument je dostupný na externích webových stránkách.
Droplet-based microfluidic chip with passive mixer for analysis with photon-upconversion nanoparticles
Mixing of the fluids in microfluidic chips is important especially in applications, where droplet contents need to be rapidly homogenized. Here, a passive method for mixing in polydimethylsiloxane ...
Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Karásek, Pavel; Horká, Marie; Planeta, Josef; Roth, Michal
2018 - anglický
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.
Klíčová slova:
supercritical water; fused silica capillary; electromigration methods
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Supercritical water in etching and morphing of siliceous surfaces for analytical separations
Applications of supercritical water in etching and morphing of siliceous surfaces for analytical separations are briefly reviewed.
Capillary electrophoresis in clinical diagnosis and monitoring
Kubáň, Petr
2018 - anglický
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in this interdisciplinary field.
Klíčová slova:
CE; CE-LIF; clinical diagnosis
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Capillary electrophoresis in clinical diagnosis and monitoring
There is a significant potential of CE to provide a useful analytical tool to analyze noninvasive samples of body fluids and stimulate the cooperation between analytical chemists and clinicians in ...
Measuring photon-upconversion luminiscence from droplets in microfluidic chips
Hlaváček, Antonín; Křivánková, Jana; Přikryl, Jan
2018 - anglický
UCNPs are lanthanide-doped nanocrystals that can be excited by near-infrared light and emit light of shorter wavelengths. Advantages of UCNPs include multiple and narrow emission bands, negligible autofluorescence and high photostability, which make UCNPs ideal luminescence label for use in droplet microfluidic. Here, we introduce the instrumentation for reading photon-upconversion luminescence of nanoparticles, which are dispersed in water droplets in a microfluidic chip.
Klíčová slova:
photon-upconversion; microfluidic chip; microdroplets; nanoparticles
Dokument je dostupný na externích webových stránkách.
Measuring photon-upconversion luminiscence from droplets in microfluidic chips
UCNPs are lanthanide-doped nanocrystals that can be excited by near-infrared light and emit light of shorter wavelengths. Advantages of UCNPs include multiple and narrow emission bands, negligible ...
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