Fairytales reinvented: How the heroines shape their own destiny in Robin McKinley's fairytale retellings Beauty, Rose Daughter and Deerskin
KONOPISKÁ, Helena
2013 - English This thesis focuses on heroines of Robin McKinley's fairy tale revisions. The introduction concerns fairy tale retellings as a genre and Robin McKinley as an author. The rest of the thesis is divided into two main parts. In the first, McKinley's retellings of "Beauty and the Beast", Beauty and Rose Daughter, are introduced. Second part of the thesis revolves around a fairy tale "Donkeyskin" that McKinley retold in her novel Deerskin. The heroines of McKinley's novels are compared with their respective counterparts from the classical versions of the fairy tale. The issue of their character traits and their agency is explored. The conclusion summarizes the findings. Tato práce se zabývá hrdinkami převyprávěných pohádek Robin McKinley. Úvod představuje převyprávěné pohádky jako žánr a Robin McKinley jako autorku. Zbytek práce je rozdělen na dvě hlavní části. Ta první pojednává o převyprávěných verzích "Krásky a Zvířete" od Robin McKinley: Beauty a Rose Daughter. Ta druhá je soustředěna na román Deerskin, což je převyprávěná verze pohádky "Oslí kůže". Hrdinky románů od McKinley jsou porovnány se svými protějšky z klasických verzí pohádek. Práce se dále zabývá jejich charaktery a jejich kontolou nad vlastními příběhy. Závěr nabízí shrnutí získaných poznatků. Keywords: Robin McKinley; pohádky; pohádka; převyprávěné pohádky; Kráska a Zvíře; Oslí kůže Available in the Digital Repository of University of South Bohemia. Fairytales reinvented: How the heroines shape their own destiny in Robin McKinley's fairytale retellings Beauty, Rose Daughter and Deerskin

This thesis focuses on heroines of Robin McKinley's fairy tale revisions. The introduction concerns fairy tale retellings as a genre and Robin McKinley as an author. The rest of the thesis is divided ...

KONOPISKÁ, Helena
Jihočeská univerzita v Českých Budějovicích, 2013

The Portrayal and Self-Image of Holden Caulfield of The Catcher in the Rye
FIBINGEROVÁ, Aneta
2013 - English The aim of the thesis is to analyze the two possible views on Holden Caulfield, the protagonist of J.D. Salinger?s The Catcher in the Rye ? his self-image and his portrayal. The self-image discussion will be based on Holden?s own perception of himself (with his statements and fantasies being the source of information), while the portrayal will be analyzed from more objective point of view. The focus of the portrayal will be the discussion of Holden?s motivation and the influence of his attitude and his family on the situation he finds himself in. Cílem práce je analýza dvou možných pohledů na Holdena Caulfielda, protagonisty románu J. D. Salingera Kdo chytá v žitě ? jeho náhledu na sebe sama a jeho vnějšího vyobrazení. Jeho sebeobraz bude analyzován na základě Holdenova vnímání sebe sama (zdrojem informací budou Holdenovy výroky a představy o jeho vlastní osobě), zatímco rozbor vnějšího vyobrazení se bude zabývat objektivními faktory ? motivací hlavního hrdiny a vliv jeho postoje a rodiny na situaci, ve které se nachází. Keywords: Holden Caulfield; dětství; dospělost; smrt; nevinnost; sebehodnocení; sebeobraz; vyobrazení; rebelství; dospívání; motivace; rodina Available in the Digital Repository of University of South Bohemia. The Portrayal and Self-Image of Holden Caulfield of The Catcher in the Rye

The aim of the thesis is to analyze the two possible views on Holden Caulfield, the protagonist of J.D. Salinger?s The Catcher in the Rye ? his self-image and his portrayal. The self-image discussion ...

FIBINGEROVÁ, Aneta
Jihočeská univerzita v Českých Budějovicích, 2013

Functional characterization of succinyl-CoA synthetase in the bloodstream form of Trypanosoma brucei
KUBIŠOVÁ, Karolína
2019 - English During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse activity of the FoF1-ATP synthase to hydrolyze ATP and pump protons into the mitochondrial intermembrane space to maintain the essential membrane potential. The dogma also states that the sole source of organellar ATP is the mitochondrial ADP/ATP carrier, which imports glycolytically derived ATP from the cytosol. In this study, we explore the possibility that the BF mitochondrion is able to contribute to its own pool of consumable ATP by utilizing an active succinyl-CoA synthase to generate ATP via substrate phosphorylation. During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse activity of the FoF1-ATP synthase to hydrolyze ATP and pump protons into the mitochondrial intermembrane space to maintain the essential membrane potential. The dogma also states that the sole source of organellar ATP is the mitochondrial ADP/ATP carrier, which imports glycolytically derived ATP from the cytosol. In this study, we explore the possibility that the BF mitochondrion is able to contribute to its own pool of consumable ATP by utilizing an active succinyl-CoA synthase to generate ATP via substrate phosphorylation. Available in the Digital Repository of University of South Bohemia. Functional characterization of succinyl-CoA synthetase in the bloodstream form of Trypanosoma brucei

During the infectious bloodstream stage (BF), the human pathogen Trypanosoma brucei lacks a canonical cytochrome-mediated respiratory chain. Therefore, this form of the parasite depends on the reverse ...

KUBIŠOVÁ, Karolína
Jihočeská univerzita v Českých Budějovicích, 2019

Interaction of gold cationic nanoparticles with blood cells and their distribution in the model organism
ŠRÁMEK, Michal
2019 - English Cationic gold nanoparticles (GNPs) are attractive candidates for drug delivery, biomedical imaging and targeted anti-cancer therapy. Due to their unique physical-chemical properties, they have great potential, especially for so-called targeted photothermal cancer therapy. Nanoparticle properties such as size, shape and surface charge play a key role in the internalization process and different types of NPs can enter cells by various pathways. It has been reported, that specific cationic gold nanorods (GNRs) preparations are taken up by both normal and cancer cells in large amounts. The nanoparticles were shown to accumulate inside vesicular structures. Prior to the start with an application of cationic GNRs in biomedicine for different kind of purposes (such as photothermal therapy, targeted drug/gene delivery carriers, fluorescence probes, pregnancy test etc.), it is necessary at first to determine their biological safety and organism's response in general. In our laboratory, we are focused mainly on the application of GNRs in photothermal therapy. That consists of cationic GNRs accumulated in tumour tissue, where they transform light into heat upon irradiation by near-infrared (NIR) light, thereby thermally damaging nearby cells. During the study, it was shown by microscopic examination of the blood that after incubation of blood with cationic GNRs in vitro, the interaction of GNRs with white blood cells and platelets was observed. Further, using histology and fluorescence microscopy it was shown that after in vivo application the cationic GNRs are mostly accumulated in mouse spleen. The obtained data contributed to an understanding of cationic GNRs distribution and toxicity at the level of the cell and organism and were published as a part of the complex study in Biomaterials journal. Kationické zlaté nanočástice (GNPs) jsou vhodnými kandidáty pro doručování léčiv, zobrazování v biomedicíně a pro cílenou léčbu nádorových onemocnění. Vzhledem k jejich unikátním fyzikálně-chemickým vlastnostem mohou být využity především pro tzv. cílenou fototermální léčbu nádorů. Během buněčné internalizace hraje klíčovou roli především velikost, tvar a povrchový náboj nanočástice. Různé typy nanočástic vstupují do buňky různými cestami. Je známo, že kationické zlaté nanotyčinky (GNRs) jsou normálními i nádorovými buňkami snadno vychytávány, přičemž jsou během endocytózy hromaděny uvnitř vezikulárních struktur. Před zahájením aplikace GNRs v biomedicíně pro různé typy účelů (jako je fototermální terapie, cílené nosiče léčiv, fluorescenční sondy, těhotenský test atd.) je nutné nejdříve určit jejich biologickou bezpečnost a reakci organismu obecně. V naší laboratoři se zaměřujeme především na aplikace GNRs ve fototermální terapii. Jejím principem je nahromadění kationických GNRs v nádorové tkáni, kde po ozáření světelným paprskem o frekvenci blízké infračervenému (NIR) světlu transformují světlo na teplo, čímž termálně poškodí buňky. Během této studie bylo ukázáno pomocí mikroskopického vyšetření krve, že po její inkubaci s kationickými GNRs in vitro dochází k interakci GNRs s bílými krvinkami a trombocyty. Dále bylo ukázáno pomocí histologie a fluorescenční mikroskopie, že po aplikaci in vivo se kationické GNRs v největší míře akumulují v myší slezině. Získaná data přispěla k porozumění distribuce a toxicity kationických GNRs na úrovni buňky a organismu a byla publikována v rámci komplexní studie v časopise Biomaterials. Keywords: zlaté nanočástice; nádorové onemocnění; fototermální terapie; krev; tkáně Available in the Digital Repository of University of South Bohemia. Interaction of gold cationic nanoparticles with blood cells and their distribution in the model organism

Cationic gold nanoparticles (GNPs) are attractive candidates for drug delivery, biomedical imaging and targeted anti-cancer therapy. Due to their unique physical-chemical properties, they have great ...

ŠRÁMEK, Michal
Jihočeská univerzita v Českých Budějovicích, 2019

Critical swimming speed in intensively cultured European perch (\kur{Perca fluviatilis} L.): Influence of fish size, production system and repeated testing
TOMÁŠEK, Ondřej
2019 - English Critical swimming speed and swimming performance in relation with different factors were tested this thesis in groups of European perch (Perca fluviatilis) with focus to utilize these results in intensive aquaculture. Thesis is consisted of four different experiments performed in swimming tunnel. In the first experiment were tested, if there are any differences in day distances of measuring between three groups of fish. Each group were measured three times. First group was measured in time (t) 0, 24 and 48 h, second group was measured in time (t) 0, 48 and 96 h. Finally, third group was measured in time (t) 0, 96 and 192 h. Second experiment was set to compare critical swimming speed in three weight categories of a fish. Weight categories of 100g, 200g and 250g were tested. In the third experiment were tested pond reared fish and aquaculture (RAS) reared fish. Results were compared together, if there are any differences. Last experiment was set to find out if there is any relationship between level of critical swimming speed and somatic indexes or fillet and caraccas yield. No one of experiments performed confirm expectations of results. In the first experiment was found that there no effect of repeated swimming tests on critical swimming speed as well as no effect of different recovery period (from previous test). In comparison of different weight categories were measured highest levels of critical swimming speed in 100g group without decreasing trend in for bigger size categories (200 and 250 g). Pond and intensively reared fish had same level in critical swimming speed. Moreover, fourth experiment did not confirm influence level of critical swimming speed to somatic indexes or fillet yield. Results of presented thesis can contribute on better technical design of rearing units for this gastronomically and economically interesting species in intensive aquaculture, mainly in RAS systems. V práci byly testovány různé faktory ve vztahu ke kritické rychlosti plavání u různých skupin okouna říčního (Perca fluviatilis) se zaměřením na využití těchto výsledků v podmínkách chovů intenzivní akvakultury. Celá práce se skládá ze čtyř různých pokusů provedených v plavacím tunelu. První pokus byl nastaven tak, aby bylo možné porovnat skupiny individuálně značených ryb, zda se liší naměřené hodnoty plavání s rozlišným odstupem opakovaných měření. Každá skupina byla změřena 3 x po sobě. První skupina byla měřena v čase (t) 0, 24 a 48 h, druhá skupina byla měřena v čase (t) 0, 48 a 96 h. Třetí skupina byla měřena v čase (t) 0, 96 and 192 h. V druhém pokuse byly porovnány rozdíly v kritické rychlosti plavání u třech váhově rozdílných skupin. Váhové kategorie 100 g, 200 g a 250 g byly testovány. Třetí pokus porovnával rozdíly kritické rychlosti plavání mezi rybami pocházejícími z intenzivní akvakultury (RAS) a rybami z rybniční akvakultury. Cílem posledního čtvrtého pokusu bylo zjistit, zda má hodnota kritické rychlosti plavání vztah k somatickým indexům nebo výtěžnosti ekonomicky zajímavých částí rybího těla. V žádném z provedených pokusů nebyly potvrzeny předešlé očekávání. U prvního experimentu bylo zjištěno, že hodnoty měřené kritické rychlosti plavání nejsou ovlivněny opakovaným provedením testu a rovněž nebyl potvrzen vliv délky zotavení z předchozího testu. Při porovnávání váhových kategorií, byly měřeny nejvyšší hodnoty kritické rychlosti plavání u 100 g skupiny, ale bez následného sestupného trendu u dalších skupin. Porovnání kritické rychlosti plavání u ryb z intenzivních a rybničních podmínek neprokázalo významné rozdíly. Rovněž bylo zjištěno, že kritické rychlosti plavání nemá vztah k somatickým indexům a výtěžnosti filet. Zjištěné a naměřené hodnoty mohou posloužit v budoucnu ke zlepšení podmínek chovu tohoto ekonomicky a gastronomicky zajímavého druhu v podmínkách intenzivní akvakultury, zejména v systémech RAS. Keywords: fitness; spotřeba kyslíku; schopnost plavání; plavací tunely; intenzivní akvakultura Available in the Digital Repository of University of South Bohemia. Critical swimming speed in intensively cultured European perch (\kur{Perca fluviatilis} L.): Influence of fish size, production system and repeated testing

Critical swimming speed and swimming performance in relation with different factors were tested this thesis in groups of European perch (Perca fluviatilis) with focus to utilize these results in ...

TOMÁŠEK, Ondřej
Jihočeská univerzita v Českých Budějovicích, 2019

The effect of CG18446 gene on the specification of circulating immune cells in \kur{Drosophila melanogaster}
RÁZKOVÁ, Anna
2018 - English Available in the Digital Repository of University of South Bohemia. The effect of CG18446 gene on the specification of circulating immune cells in \kur{Drosophila melanogaster}

RÁZKOVÁ, Anna
Jihočeská univerzita v Českých Budějovicích, 2018

Polysemy in English Modal Verbs
KUBÁSEK, Vít
2019 - English One of the features of modal verbs is their polysemous meaning. The goal of this bachelor thesis is to quantify polysemy by means of spoken (BASE) and written (ukWaC) corpus. Modal meanings of the selected English modal verbs are classified based on available sources, mainly on Cognitive English Grammar. The thesis also contains prototypical examples of all modal meanings of each selected modal verb. Jedním z rysů modálních sloves je, že jsou polysémní. Cílem této bakalářské práce je kvantifikovat tuto polysémii za pomoci mluveného (BASE) a psaného (ukWaC) korpusu. Modální významy vybraných anglických modálních sloves jsou klasifikovány na základě dostupné odborné literatury, zejména pak Cognitive English Grammar. Práce také obsahuje prototypické příklady jednotlivých druhů modalit u vybraných modálních sloves. Keywords: modální slovesa; modalita; polysémie; korpusová analýza Available in the Digital Repository of University of South Bohemia. Polysemy in English Modal Verbs

One of the features of modal verbs is their polysemous meaning. The goal of this bachelor thesis is to quantify polysemy by means of spoken (BASE) and written (ukWaC) corpus. Modal meanings of the ...

KUBÁSEK, Vít
Jihočeská univerzita v Českých Budějovicích, 2019

The role of mitochondrial SCoAS substrate-level phosphorylation in the bloodstream form \kur{T. brucei}
HUSOVÁ, Michaela
2019 - English Mitochondrial metabolism of Trypanosoma brucei is considered highly reduced because of its dysfunctional electron transport chain and tricarboxylic acid cycle. But previously published paper suggests significant mitochondrial ATP pool created by substrate phosphorylation via succinyl coenzyme A synthetase, which plays crucial role in T. brucei survival. This thesis is therefore focused on substrate phosphorylation and on influence of succinyl coenzyme A synthetase heterodimer RNAi on T. brucei cells. Mitochondrial metabolism of Trypanosoma brucei is considered highly reduced because of its dysfunctional electron transport chain and tricarboxylic acid cycle. But previously published paper suggests significant mitochondrial ATP pool created by substrate phosphorylation via succinyl coenzyme A synthetase, which plays crucial role in T. brucei survival. This thesis is therefore focused on substrate phosphorylation and on influence of succinyl coenzyme A synthetase heterodimer RNAi on T. brucei cells. Keywords: Trypanosoma brucei; mitochondria; succinyl coenzyme A synthetase; substrate phosphorylation; RNAi Available in the Digital Repository of University of South Bohemia. The role of mitochondrial SCoAS substrate-level phosphorylation in the bloodstream form \kur{T. brucei}

Mitochondrial metabolism of Trypanosoma brucei is considered highly reduced because of its dysfunctional electron transport chain and tricarboxylic acid cycle. But previously published paper suggests ...

HUSOVÁ, Michaela
Jihočeská univerzita v Českých Budějovicích, 2019

Trehalose as an important energy source for immune cells in Drosophila melanogaster
LEHR, Katharina
2019 - English The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by Leptopilina boulardi. Moreover, the energy supply was shut down by deactivating the trehalose transporter and measuring the lamellocyte production, again in uninfected and infected larva to test the role of trehalose for the selfish immune system. The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by Leptopilina boulardi. Moreover, the energy supply was shut down by deactivating the trehalose transporter and measuring the lamellocyte production, again in uninfected and infected larva to test the role of trehalose for the selfish immune system. Available in the Digital Repository of University of South Bohemia. Trehalose as an important energy source for immune cells in Drosophila melanogaster

The aim of this thesis was the identification of the change of trehalase gene expression in hemocytes of Drosophila melanogaster in uninfected and infected larvae using parasitoid wasp infection by ...

LEHR, Katharina
Jihočeská univerzita v Českých Budějovicích, 2019

Role of the serotonin receptor type 7 (5-HT7) in autoimmune disease
NUßBAUMER, Mia
2023 - English Serotonin receptor type 7 (5-HT7) displays immunomodulatory functions and is overexpressed in individuals with Crohn's disease. Here, we set out to determine 5-HT7 mode of interaction with its cognate proteins Gs and G12. A unique type of coupling, termed 'inverse coupling' is known to occur between the 5-HT7 receptor and the Gs protein. The aim of our research was to determine whether G12 can also undergo this kind of coupling. We found that the G12 protein has a surprisingly lower mobility than Gs implying a specific interaction with a membrane domain or another membrane-localized protein. For both G proteins, we were unable to detect their inverse coupling with the 5-HT7 receptor at room temperature. Our findings indicate peculiar differences in G protein mobility and emphasize the importance of temperature for studies of interactions between G proteins and G protein-coupled receptors. Serotonin receptor type 7 (5-HT7) displays immunomodulatory functions and is overexpressed in individuals with Crohn's disease. Here, we set out to determine 5-HT7 mode of interaction with its cognate proteins Gs and G12. A unique type of coupling, termed 'inverse coupling' is known to occur between the 5-HT7 receptor and the Gs protein. The aim of our research was to determine whether G12 can also undergo this kind of coupling. We found that the G12 protein has a surprisingly lower mobility than Gs implying a specific interaction with a membrane domain or another membrane-localized protein. For both G proteins, we were unable to detect their inverse coupling with the 5-HT7 receptor at room temperature. Our findings indicate peculiar differences in G protein mobility and emphasize the importance of temperature for studies of interactions between G proteins and G protein-coupled receptors. Keywords: G-protein; G protein-coupled receptor; cell signaling; serotonin; microscopy; single-molecule imaging; inflammation Available in the Digital Repository of University of South Bohemia. Role of the serotonin receptor type 7 (5-HT7) in autoimmune disease

Serotonin receptor type 7 (5-HT7) displays immunomodulatory functions and is overexpressed in individuals with Crohn's disease. Here, we set out to determine 5-HT7 mode of interaction with its cognate ...

NUßBAUMER, Mia
Jihočeská univerzita v Českých Budějovicích, 2023