Number of found documents: 577
Published from to

Proceedings of 24th School of Mass Spectrometry
Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
2023 - Czech
Keywords: mass spectrometry; LC-MS; GC-MS Available at various institutes of the ASCR
Proceedings of 24th School of Mass Spectrometry

Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
Ústav organické chemie a biochemie, 2023

Biopharmaceuticals from the MS point of view
Hubálek, Martin; Lenčo, J.
2023 - Czech
The course aims to introduce biotherapeutics, which are becoming an increasingly important part of the pharmacotherapy of serious diseases. Mass spectrometry plays an important role for their quality control in the pharmaceutical industry. We will introduce the audience to biopharmaceuticals, which in this context are peptides, proteins, monoclonal antibodies or oligonucleotides. From the analyst's point of view, we will focus mainly on antibody drugs, looking at the possibilities of their separation and analysis by mass spectrometry, at the level of intact protein molecules, antibody subunits or peptides. Kurz má za cíl představit bioléčiva, která se stávají stále významnější součástí farmakoterapie závažných onemocnění. Pro kontrolu jejich kvality ve farmaceutickém průmyslu hraje důležitou roli hmotnostní spektrometrie. Uvedeme posluchače do problematiky bioléčiv, kterými jsou v tomto pohledu peptidy, proteiny, monoklonální protilátky nebo oligonukleotidy. Z pohledu analytika se zaměříme především na protilátková léčiva, podíváme se na možnosti jejich separace a analýzy pomocí hmotnostní spektrometrie, a to na úrovni intaktních proteinových molekul, protilátkových podjednotek nebo peptidů. Keywords: mass spectrometry; biopharmaceuticals; LC-MS Available on request at various institutes of the ASCR
Biopharmaceuticals from the MS point of view

The course aims to introduce biotherapeutics, which are becoming an increasingly important part of the pharmacotherapy of serious diseases. Mass spectrometry plays an important role for their quality ...

Hubálek, Martin; Lenčo, J.
Ústav organické chemie a biochemie, 2023

24th School of Mass Spectrometry. Course D - LC-MS Troubleshooting
Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
2023 - Czech
Keywords: mass spectrometry; liquid chromatography; troubleshooting Available on request at various institutes of the ASCR
24th School of Mass Spectrometry. Course D - LC-MS Troubleshooting

Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
Ústav organické chemie a biochemie, 2023

Fundamentals of proteomics
Harant, K.; Křenková, Alena
2023 - Czech
The first part of the course will be an introduction to proteomics with the aim to show current methods and tools used for proteomic data acquisition and evaluation. In the first part, we will discuss in abbreviated form all steps of the experimental procedure from 1) designing the experiment to be suitable for subsequent LC/MS analysis, 2) sample preparation using examples of immunoprecipitation and complex lysate, to 3) measurement options using Data Dependent or Data Independent acquisition. The course will also include a critical evaluation of the quality of the measured data. The evaluation will focus on the possibilities of data processing in freeware software such as MaxQuant and DIAnn. We will also briefly introduce you to commercial programs - Proteome Discoverer, PEAKS, Spectronaut.The second part of the course will include an introduction to statistical processing of quantitative proteomic data in Perseus software. Emphasis will be placed on understanding important statistical concepts (data distribution, t-test, ANOVA, p-value, FDR, etc.) and understanding all necessary steps of statistical analysis such as data filtering, data normalization, statistical testing, etc. There will be model data that participants will process during the course. Náplní první části kurzu bude úvod do proteomiky s cílem ukázat současné metody a nástroje použitelné k akvizici a vyhodnocení proteomických dat. V první části probereme ve zkrácené formě všechny kroky experimentálního postupu od 1) designu experimentu tak, aby byl vhodný pro následnou LC/MS analýzu, 2) přípravy vzorku na příkladech imunoprecipitace a komplexního lyzátu až po 3) možnosti měření pomocí Data Dependent nebo Data Independent acquisition. Součástí kurzu bude i kritické hodnocení kvality naměřených dat. Při vyhodnocení se zaměříme na možnosti zpracování dat ve freeware SW typu MaxQuant a DIAnn. Rovněž Vás stručně seznámíme s komerčními programy – Proteome Discoverer, PEAKS, Spectronaut.Druhá část kurzu bude zahrnovat úvod do statistického zpracování kvantitativních proteomických dat v softwaru Perseus. Důraz bude kladen na pochopení důležitých statistických pojmů (distribuce dat, t-test, ANOVA, p-value, FDR, atd.) a porozumění všem nezbytným krokům statistické analýzy, jako je filtrace dat, normalizace dat, statistické testování, apod. K dispozici budou modelová data, která budou účastníci v rámci kurzu zpracovávat. Keywords: mass spectrometry; proteomics; data analysis Available on request at various institutes of the ASCR
Fundamentals of proteomics

The first part of the course will be an introduction to proteomics with the aim to show current methods and tools used for proteomic data acquisition and evaluation. In the first part, we will discuss ...

Harant, K.; Křenková, Alena
Ústav organické chemie a biochemie, 2023

24th School of Mass Spectrometry. Course B - Fundamentals of ICP-MS
Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
2023 - Czech
Keywords: mass spectrometry; ICP-MS; speciation analysis Available on request at various institutes of the ASCR
24th School of Mass Spectrometry. Course B - Fundamentals of ICP-MS

Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
Ústav organické chemie a biochemie, 2023

24th School of Mass Spectrometry. Course A - EI spectra interpretation
Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
2023 - Czech
Keywords: mass spectrometry; EI; data analysis Available on request at various institutes of the ASCR
24th School of Mass Spectrometry. Course A - EI spectra interpretation

Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
Ústav organické chemie a biochemie, 2023

24th School of Mass Spectrometry. Course C - Fundamentals of proteomics
Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
2023 - Czech
Keywords: mass spectrometry; proteomics; data analysis Available on request at various institutes of the ASCR
24th School of Mass Spectrometry. Course C - Fundamentals of proteomics

Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
Ústav organické chemie a biochemie, 2023

Abstracts of lectures in the “Mládí vpřed“ section and poster presentations of 24th School of Mass Spectrometry
Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
2023 - Czech
Keywords: mass spectrometry; LC-MS; GC-MS Fulltext is available at external website.
Abstracts of lectures in the “Mládí vpřed“ section and poster presentations of 24th School of Mass Spectrometry

Cvačka, Josef; Vlk, Mikuláš; Vrkoslav, Vladimír; Hubálek, Martin
Ústav organické chemie a biochemie, 2023

NIVB Meeting 2023
Hostomský, Zdeněk; Vácha, R.; Pichová, Iva; Šímová, Šárka
2023 - English
NIVB Meeting 2023 – the second meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora on 2. 10. – 5. 10. 2023 will be again after one year an opportunity to meet the participating teams, discuss excellent science, establish collaborations, and present the progress of the project to the members of the International Scientific Advisory Board. Thanks to funding from the Czech Economic Recovery Plan, a number of important results have already been published in high impact journals. Several networking workshops have been organized and scientific collaborations have been established. The meeting in Kutná Hora is therefore a summary of all that has happened in the project over the past year. The main goal of the NIVB is to facilitate cooperation between 30 participating research teams from 8 Czech research institutions, and this goal is gradually being achieved. The NIVB acts as a common communication platform to discuss the desired cooperation of the NIVB research teams, especially those that have not had much contact so far due to their inter-institutional, interdisciplinary or inter-regional distance.\nWe thank all the participants who contributed to the meeting with 30 oral presentations and 67 posters. The NIVB 2022 meeting had the ambition to launch a new series of annual meetings dedicated to virology and bacteriology, and NIVB 2023 fulfils this ambition and continues to aim to inform the wider scientific community about new developments, trends and issues in these disciplines. Keywords: virology; bacteriology; National Institute of Virology and Bacteriology (NIVB); NIVB meeting Available in digital repository of the ASCR
NIVB Meeting 2023

NIVB Meeting 2023 – the second meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora on 2. 10. – 5. 10. 2023 will be again after one year an opportunity to meet the ...

Hostomský, Zdeněk; Vácha, R.; Pichová, Iva; Šímová, Šárka
Ústav organické chemie a biochemie, 2023

EI spectra interpretation
Polášek, Miroslav
2023 - Czech
Introduction to the interpretation of mass spectra. Basic mechanisms of fragmentation reactions in electron ionization. An intensive exercise on selected examples of mass spectra of electron ionization of different classes of organic compounds, which will be carried out in the spirit of the elementary didactic principle of progression from simple to complex with maximum emphasis on the independent work of the participants.\n\n Úvod do problematiky interpretace hmotnostních spekter. Základní mechanizmy fragmentačních reakcí při elektronové ionizaci. Intenzivní cvičení na vybraných příkladech hmotnostních spekter elektronové ionizace různých tříd organických sloučenin, které bude probíhat v duchu elementární didaktické zásady postupu od jednoduchého ke složitějšímu s maximálním důrazem na samostatnou práci účastníků. Keywords: mass spectrometry; EI; data analysis Available at various institutes of the ASCR
EI spectra interpretation

Introduction to the interpretation of mass spectra. Basic mechanisms of fragmentation reactions in electron ionization. An intensive exercise on selected examples of mass spectra of electron ...

Polášek, Miroslav
Ústav organické chemie a biochemie, 2023

About project

NRGL provides central access to information on grey literature produced in the Czech Republic in the fields of science, research and education. You can find more information about grey literature and NRGL at service web

Send your suggestions and comments to nusl@techlib.cz

Provider

http://www.techlib.cz

Facebook

Other bases